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In-Silico Evaluation of the Capsid Proteins of FMDV as Potential Vaccine Candidates

Received: 23 November 2014     Accepted: 2 March 2015     Published: 9 March 2015
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Abstract

In this study, the capsid proteins of four major serotypes of Foot and Mouth Disease Virus (FMDV) were assessed as the vaccine candidates. Different protein sequences regarding FMDV capsid of O, A, Asia 1 and C type were identified from NCBI Genome Database and UniprotKB. Phylogenetic tree of the four serotypes was developed using ClustalW software. HMMTOP, RANKPEP, Swiss-Model and Vaxign software were used for comparing the capsid proteins in terms of their feasibility as vaccine candidates. The virus and viral serotype were identified from the cultured disease sample using RT-PCR. Our results revealed that different capsid proteins of the four serotypes vary in their suitability to be considered as peptide vaccine components. Viral protein 1 (VP1) for Asia 1 serotype represented the best result as a vaccine candidate. The VP1 region of Asia 1 serotype amplified based on the result of dry lab analysis. Our findings provide a future indication of multivalent vaccine development against FMDV.

Published in Computational Biology and Bioinformatics (Volume 3, Issue 1)
DOI 10.11648/j.cbb.20150301.12
Page(s) 6-20
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Reverse Vaccinology, FMDV, Capsid Protein, Viral Protein 1, Vaccine Candidate

References
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Cite This Article
  • APA Style

    F. M. N. Hassan, Md. Shaifur Rahman, K. M. T. Rahman, Sharmin S. Sumi, Md. F. Islam, et al. (2015). In-Silico Evaluation of the Capsid Proteins of FMDV as Potential Vaccine Candidates. Computational Biology and Bioinformatics, 3(1), 6-20. https://doi.org/10.11648/j.cbb.20150301.12

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    ACS Style

    F. M. N. Hassan; Md. Shaifur Rahman; K. M. T. Rahman; Sharmin S. Sumi; Md. F. Islam, et al. In-Silico Evaluation of the Capsid Proteins of FMDV as Potential Vaccine Candidates. Comput. Biol. Bioinform. 2015, 3(1), 6-20. doi: 10.11648/j.cbb.20150301.12

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    AMA Style

    F. M. N. Hassan, Md. Shaifur Rahman, K. M. T. Rahman, Sharmin S. Sumi, Md. F. Islam, et al. In-Silico Evaluation of the Capsid Proteins of FMDV as Potential Vaccine Candidates. Comput Biol Bioinform. 2015;3(1):6-20. doi: 10.11648/j.cbb.20150301.12

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  • @article{10.11648/j.cbb.20150301.12,
      author = {F. M. N. Hassan and Md. Shaifur Rahman and K. M. T. Rahman and Sharmin S. Sumi and Md. F. Islam and Md. Badrul Alam and Md. Giasuddin and Khondoker M. Hossain},
      title = {In-Silico Evaluation of the Capsid Proteins of FMDV as Potential Vaccine Candidates},
      journal = {Computational Biology and Bioinformatics},
      volume = {3},
      number = {1},
      pages = {6-20},
      doi = {10.11648/j.cbb.20150301.12},
      url = {https://doi.org/10.11648/j.cbb.20150301.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbb.20150301.12},
      abstract = {In this study, the capsid proteins of four major serotypes of Foot and Mouth Disease Virus (FMDV) were assessed as the vaccine candidates. Different protein sequences regarding FMDV capsid of O, A, Asia 1 and C type were identified from NCBI Genome Database and UniprotKB. Phylogenetic tree of the four serotypes was developed using ClustalW software. HMMTOP, RANKPEP, Swiss-Model and Vaxign software were used for comparing the capsid proteins in terms of their feasibility as vaccine candidates. The virus and viral serotype were identified from the cultured disease sample using RT-PCR. Our results revealed that different capsid proteins of the four serotypes vary in their suitability to be considered as peptide vaccine components. Viral protein 1 (VP1) for Asia 1 serotype represented the best result as a vaccine candidate. The VP1 region of Asia 1 serotype amplified based on the result of dry lab analysis. Our findings provide a future indication of multivalent vaccine development against FMDV.},
     year = {2015}
    }
    

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  • TY  - JOUR
    T1  - In-Silico Evaluation of the Capsid Proteins of FMDV as Potential Vaccine Candidates
    AU  - F. M. N. Hassan
    AU  - Md. Shaifur Rahman
    AU  - K. M. T. Rahman
    AU  - Sharmin S. Sumi
    AU  - Md. F. Islam
    AU  - Md. Badrul Alam
    AU  - Md. Giasuddin
    AU  - Khondoker M. Hossain
    Y1  - 2015/03/09
    PY  - 2015
    N1  - https://doi.org/10.11648/j.cbb.20150301.12
    DO  - 10.11648/j.cbb.20150301.12
    T2  - Computational Biology and Bioinformatics
    JF  - Computational Biology and Bioinformatics
    JO  - Computational Biology and Bioinformatics
    SP  - 6
    EP  - 20
    PB  - Science Publishing Group
    SN  - 2330-8281
    UR  - https://doi.org/10.11648/j.cbb.20150301.12
    AB  - In this study, the capsid proteins of four major serotypes of Foot and Mouth Disease Virus (FMDV) were assessed as the vaccine candidates. Different protein sequences regarding FMDV capsid of O, A, Asia 1 and C type were identified from NCBI Genome Database and UniprotKB. Phylogenetic tree of the four serotypes was developed using ClustalW software. HMMTOP, RANKPEP, Swiss-Model and Vaxign software were used for comparing the capsid proteins in terms of their feasibility as vaccine candidates. The virus and viral serotype were identified from the cultured disease sample using RT-PCR. Our results revealed that different capsid proteins of the four serotypes vary in their suitability to be considered as peptide vaccine components. Viral protein 1 (VP1) for Asia 1 serotype represented the best result as a vaccine candidate. The VP1 region of Asia 1 serotype amplified based on the result of dry lab analysis. Our findings provide a future indication of multivalent vaccine development against FMDV.
    VL  - 3
    IS  - 1
    ER  - 

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Author Information
  • Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh

  • Tissue Banking and Biomaterial Research Unit, Atomic Energy Research Establishment, BAEC, Dhaka-1349, Bangladesh

  • Research and Development Division, Incepta Vaccine Limited, Dhaka, Bangladesh

  • Department of Biochemistry, University of Alberta,Edmonton, Alberta, Canada T6G 2H7

  • Department of Biochemistry, University of Saskatchewan, Saskatoon, S7N 5E5 Canada

  • Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh

  • Bangladesh Livestock Research Institute (BLRI), Savar, Dhaka -1341, Bangladesh

  • Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh

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